詹妮弗·莫法特博士

ASSOCIATIONS / MEMBERSHIPS

研究抽象

大多数的美国人.S. have had chicken pox, caused by the varicella zoster virus (VZV), or have received the vaccine. This virus remains latent in the body for life, and can reactivate as the disease called shingles. Learning how VZV interacts with human cells is the major goal of our research, and we hope to use this knowledge to develop new drug treatments and to improve the vaccine.

Current work in my lab is focused on the cell functions that are required for VZV replication. 具体地说, we are looking at how VZV dysregulates cell cycle proteins, what effects VZV has on cellular DNA synthesis, and how VZV impacts cell-signaling kinase cascades. To study these aspects of virology and cell biology, we have used chemical inhibitors of cellular kinases to understand what enzymes are needed for VZV to grow. We found that a cell cycle kinase inhibitor, roscovitine, is highly potent against VZV replication, 病毒基因转录, 蛋白质表达. Other compounds that target the cell are being studied for their antiviral properties.

Other tools that we use to study VZV are virus mutants, a 鼠标 model of virus replication, 皮肤器官培养. 使用人体皮肤, either grown in culture or implanted into SCID mice, we have identified genes that are necessary for this virus to replicate in skin. Since chicken pox and shingles are infamous for itchy, 疼痛的皮肤病变, this is a very important system for studying VZV. Creating mutant viruses to study in the skin model has been slow, but molecular techniques exist, and new ones are being developed, to analyze the contribution of individual VZV genes to pathogenesis.

选择引用

詹妮弗·F·莫法特., 利·泽博尼，保罗·R. Kinchington, Charles Grose, Hideto Kaneshima, 和安·M. 阿尔文. 1998. Attenuation of the vaccine Oka strain of varicella-zoster virus and the role of glycoprotein C in alphaherpesvirus virulence demonstrated in the SCID-hu 鼠标. 病毒学杂志, 72:965-974.

詹妮弗·F·莫法特., Leigh Zerboni, Marvin H. 大梁, 托马斯·C. 海涅曼，杰弗里，我. Cohen, Hideto Kaneshima, 和安·M. 阿尔文. 1998. 的 ORF47 and ORF66 putative protein kinases of varicella-zoster virus determine tropism for human T cells and skin in the SCID-hu 鼠标. Proceedings of the National Academy of 科学[j] .中国生物医学工程学报，25 (5):11969-11974.

詹妮弗·F·莫法特. 和安·M. 阿尔文. 1999. 水痘一带状疱疹病毒 infection of T cells and skin in the SCID-hu 鼠标 model. In Handbook of Animal Models of Infection, pp. 973-979. 学术出版社，伦敦.

理查德·桑托斯.克里斯托弗·C. 本杰明·P·哈特菲尔德. 乔治·法加. 南希·L·帕迪拉. 科尔, 詹妮弗·F. 莫法特安·M. 阿尔文, 威廉T. Ruyechan, John Hay, and Charles Grose. 2000. 水痘一带状疱疹病毒 gE escape mutant VZV-MSP: a novel genotype with an accelerated cell-to-cell spread phenotype in both infected cell cultures and SCID-hu mice. 病毒学, 275:306-317.

詹妮弗·F·莫法特., Hideki Ito, Marvin 大梁, Shannon 泰勒和Ann M. 阿尔文. 2002. Glycoprotein I of varicella zoster virus is required for viral replication in skin and T cells. 病毒学杂志, 76:8468-8471.

贝瑟J. 大梁MH. Zerboni L. Bagowski CP. Ito H. 莫法特 J. Ku CC. 阿尔文是. 2003. 分化的 varicella-zoster virus ORF47 protein kinase and IE62 protein binding domains and their contributions to replication in human skin xenografts in the SCID-hu 鼠标. 病毒学杂志, 77:5964-74.

泰勒, Shannon L, Paul R. Kinchington, Andrew Brooks, and 珍妮花 F. 莫法特. 2003. 的 cyclin-dependent kinase inhibitor roscovitine prevents varicella zoster virus replication and DNA synthesis. 病毒学杂志, 78:2853-2862.

詹妮弗·F·莫法特., 斯泰西一. Leisenfelder, Michelle A. McMichael, and Shannon L. 泰勒. 2004. Viral and cellular kinases are potential antiviral targets and have a central role in varicella zoster virus pathogenesis. Biochimica et Biophysica Acta, in press.